Laboratory of the Bacterial Animal Diseases

LABORATORY OF THE BACTERIAL ANIMAL DISEASES

 

 

1976-1981 – Education at the Ukrainian Agricultural Academy, Faculty of Veterinary Medicine, Kiev. Diploma of Veterinary Doctor.

2000 – Ph.D. Ukrainian Academy of Agricultural Sciences

2010 – Present – leader of Department of Bacterial animal diseases and control of the VIP (Veterinary immunological preparations) (earlier – associative infection) of the Institute of Veterinary Medicine of Ukrainian National Academy Agricultural Science, c. Kiev manager of the research Program, bacteriologist

2003 – 2010 – leader researcher of the Institute of Veterinary Medicine of Ukrainian Academy Agricultural Science, c. Kiev, microbiologist.

2002-2003 – leader of Department of National Center of the Strains of Microorganisms – State Scientific – Control Institute of Biotechnology and Strains of Microorganisms, c. Kiev

1996 – 2002 – Director of the Laboratory of the Veterinarian – sanitatation expertise in the market, c. Kiev

1990 -1996 – Director of the Productive Veterinarian Laboratory in the pigfarm «Kalityansky» (production – 108 thousand pigs in year) Kiev’s region

1981-1990 – veterinary doctor – bacteriologist of the Productive Veterinarian Laboratory in the pigfarm «Kalityansky» ( production – 108 thousand pigs in year) Kiev’s region

Field of Expertise – Leading specialist in Ukraine in swine respiratory infections diagnostics, author of new methods and sets to diagnose swine respiratory author of 2 standards, 3 patents, 44 publications Author of several new veterinary vaccines and technologies for Veterinary Medicine.

History of the laboratory

Department of Bacterial animal diseases and control of the VIP (Veterinary immunological preparations) (earlier – associative infections) of the Institute of Veterinary Medicine was founded in 1977 in Kiev as the laboratory of swine diseases. The main goal of the laboratory is animal infectious pathology study and creation and improvement of new diagnostic kits, biological preparations to protect animal populations from dangerous and especially dangerous pathogens.

The first Leader of Department (1977- 2010) was Ph.D. Pavlov Yevgeniy Grigorovich – the famous scientist – epizootologist, bacteriologist and specialist by swine diseases.

The laboratory staff

Ayshpur Olena – Head of the Laboratory

Mushtuk Irina – Senior Researcher

Huhley Mariya – technical worker

Sheremet Nataliya  – veterinary doctor

Kovalchuk Marina  – technical worker

The main directions of research works

The main directions of research works of laboratory of the Bacterial animal diseases and control of the VIP were and remain such :

• study and development of theoretical and practical aspects of infectious pathology of pigs;
• creation of specific preventives preparations against the most widespread bacillosiss of pigs;
• a grant of consultative and practical help to the pigfarms in the questions of prophylaxis and treatment of swine diseases.
  

The main scientific achievements of the laboratory

As a result of implementation of research works for practice such immunobiological preparations were worked out :

• a vaccine against colibacteriosis of piglets from the local strains of E.coli;
• a vaccine against Glasser’s disease of pigs (1992) ;
• associated vaccine against Glasser’s disease and salmonellosis of pigs (1998) ;
• vaccine “Haemoenterotoxal” – associative vaccines porcine Glasser’s disease, salmonellosis and edema disease (2006-2012).

In pigfarm at presence of 15 thousand pigs on condition of the associated virus-bacterial motion of respiratory swine diseases of application of “Haemoenterotoxal” – associative vaccines porcine Glasser’s disease, salmonellosis and edema disease of the laboratory of the associated infections worked out by employees (E.G.Pavlov, O.Y.Ayshpur, J.G Pavlova) assisted the stop of epizootic process. For period of the use of vaccine general departure of piglets went down twice. Additionally among vaccine animals almost 700 pigs are stored. At that pigfarm got a sale more than 400 thousand hrn., that in many times exceeds expenses on a vaccine.

In the pigfarm on 10 000 sows diagnostic studies were undertaken on determination of reason of the complex of respiratory diseases of pigs. The educed etiologic factors became basis for instituting control above these diseases. In the chart of curative and prophylactic treatments of animals preparations were used worked out by the employees of our laboratory.

Works of laboratory are protected by 5 certificates, one patent of the USSR, by the 3 patents of Ukraine.

In our laboratory museum are stored and maintained more than 100 strains of bacterias, such as:

• E.coli
• Mycoplasma hyopneumoniae, Mycoplasma hyorginis
• choleraesuis, Salm. typhymurium, Salm. pullorum gallinarum
• Pasteurella multocida Pasteurella haemolitica
• Bordetella bronchiseptica
• Proteus mirabilis, Proteus vulgaris
• A.pleurophneumoniae
• H.parasuis
• Streptococcus spp., Staphylococcus aureus
• Enterobacter spp.
• Citrobacter spp.
• Klebsiella pneumonia

International collaboration

• Participation in 20^th International Pig Veterinary Society Congress, 2008, Durban, South Africa (Poster Paper).
• Meeting with researcher of the Pacific Northwest National Laboratory, Richland, USA, 2008.
• Participation in International Conference Veterinary Laboratory Agency, London, 2009.
• Participation in the 2014 American Society for Microbiology Biodefense and Emerging Diseases Research Meeting, January 27-29, 2014, Washington, USA (Poster Paper).
• Participation in the American Society for Microbiology 114^th General Meeting, May 17-20, 2014, Boston, USA (Poster Paper).

Collaboration in Ukraine

Over the years, we have worked with such farms and corporation:

• «Slobozhanskiy» Kharkiv region
• «Bahmutskiy Agrarniy Soyuz» Donezk region
• «Liga» Kirovograd region
• «Chornobaymyaso» Cherkasy region
• «Kalita» Kiev region
• «Trubizh» Kiev region
• «Agro-Elita» Dnipropetrovsk region
• «Novogrigorivskiy» Zaporizhye region
• «Start» Hmelnizk region
• «Maryanovskiy» Cherkasy region
• Corporation «Arterium»
• Corporation «Ceva»
• Corporation «Brovarfarma»
  

The most significant publications

№	Title	Journal, Edition, Magazine (number, year) or patent specifications	Number of printed sheets or pages	Names of authors
1.	The production and control of quality of the veterinary immunobiological preparations in Ukraine	Ukrainian Veterinary medicine-2003. -№8. -P.19-20.	2	O.Y.AyshpurA.M.Golovko
2.	Mycoplasmas. The problems of the contamination of the biopreparations.	“Veterinary biotechnology”.-2004. -№5. P..3-6.	4	O.Y.Ayshpur
3.	The use of the hyperimmunized sera on the basis of local strains of bacterial infections agents.	“Veterinary biotechnology”.-2005. -№6. -P.36-40.	5	O.Y.AyshpurO.O.Salganska.
4.	Haemoenterotoxal – associative  porcine glasser’s disease salmonellosis and edema disease vaccine.	“Veterinary biotechnology”.-2006. -№9. -P.204-212.	9	O.Y.AyshpurE.G.Pavlov,J.G Pavlova O.O.Petechel
5.	The results of monitoring of the respiratory swine diseases.,	“Veterinary biotechnology”.-2007. -№10. -P.3-11.	9	O.Y.AyshpurY.G.Pavlov
6.	Proceeding of circovirus associated infection of swines (first message)	«Veterinary biotechnology».-2007. -№11. -P.163-167.	5	O.Y.AyshpurO.N.Deryabin, Y.G.Pavlov
7.	The efficacy of the gaemoenterotoxal during the acute current of swine respiratory diseases in the industrial pigfarm	«Veterinary biotechnology».-2008. -№12. -P.9-12.	4	O.Y.AyshpurY.G.PavlovM.O.Sidoruk
8.	The resistance of the agents of swine respiratory diseases to the antibacterial preparations	«Veterinary biotechnology».-2008. -№12. -P.13-18.	5	O.Y.AyshpurY.G.PavlovN.V.Sapon.
9.	Essential oils as an alternative to antibiotics in respiratory infections treatment and prophylaxis in pigs	Proceedings of the 20th Congress, Durban, South Africa, 22-26 June 2008. Vol.2 Poster Papers	1	O.Y.AyshpurA.F.ObrazgeiV.G.KvachovV.P.Sapeiko
10.	The particularities of manifestations of the pathologic processes in the swine lungs during the respiratory diseases	«Veterinary biotechnology».-2008. -№12. -P.162-165.	4	O.Y.AyshpurY.G.Pavlov
11.	The results of monitoring of the swine mycoplasmos.	«Veterinary biotechnology».-2008. -№13. -P.167-169.	3	O.Y.AyshpurY.G.Pavlov
12.	The  results of the immune control of associative diseases pulmonary mycoplasmosis and actinobacillus pleuropneumoniae.	«Veterinary biotechnology».-2009. -№14. -P.12-14.	3	O.Y.AyshpurE.G.Pavlov, O,P.Ivanenko
13.	Definition  of the adhesive activity of microorganisms by the erythrocytic  model.	«Veterinary biotechnology».-2009. -№14. -P.198-203.	6	O.Y.AyshpurE.G.Pavlov
14.	The sum data of the serological monitoring of the bacterial swine respiratory diseases.	«Veterinary biotechnology».-2009. -№15. -P.12-16.	5	O.Y.AyshpurY.G.Pavlov, Y.G.Pavlova, N.V.Sapon, O.P.Ivanenko
15.	The proliferative enteropathy (pe) in pigfarms.	«Veterinary biotechnology».-2009. -№15. -P.285-290.	6	O.Y.AyshpurY.G.Pavlov, N.V.Sapon, O.V.Lozhkina,I.G.Kalinovska
16	Role of bioassays during the studing of pathogenesis of swine bacterial pneumonias.	«Veterinary Medicine. -2011. – Kharkov. – P.275-276	2	O.Y.Ayshpur

Patents

3 Patents of Ukraine is the protection of author’s right.

1. A methodof manufacturing of the Gaemoenterotoxal – associative porcine Glasser’s disease Salmonellosis and Oedema disease vaccine.
2. Strain of the bacterium coli О157:К-88 for manufacturing of the vaccine against piglets colibacillosis.
3. A methodof manufacturing of the inactivating polyvalent vaccine against animal colibacillosis from the localstrainsof the pathogen.

Grants

2006 – 2008 –the Project of STCU with collaborator Dr. Weller (Pacific Northwest National Laboratory, USA) Project Title: Development of Veterinary Preparations Based on Plant Essential Oils as Alternative to Antibiotics and Chemicals

Development of new antimicrobial preparations including components of essential plant oils in frames of STCU Project 3553 according to U.S. Government IPP Program, PNNL T2-274, development of new veterinary vaccines.

Ayshpur O.Y. – Manager of Project, Ph.D., Researcher, Specialist in swine respiratory infections diagnostics, author of new methods and test kits for swine respiratory infections diagnose.