Research Training Center for Animal Disease Diagnostics

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Halka Ihor Vasyliovych (specialty 16.00.03 – veterinary microbiology, epizootology, infectious diseases and immunology). Phone: +38-050-546-40-47; E-mail: ptica2005@ukr.net

Laboratory History

The center was built under the “Biological Threat Reduction Program” and US Agreement on cooperation in preventing the proliferation of technology, pathogens and expertise that could be used in the development of biological weapons. In October 2010 the State Committee of Veterinary Medicine of Ukraine, National Academy of Agrarian Sciences of Ukraine and the Department of Threat Reduction in Kyiv, and the United States Embassy in Ukraine signed a memorandum on technical assistance for the “Reduction of biological threat in Ukraine” project.

Institute of Veterinary Medicine received technical assistance in the form of established and financed by the American side of the Training center and teaching laboratory Biosafety Level BSL-2 with a conference hall. Refurbished and equipped 623 m², installed ventilation system, generator backup power, sophisticated diagnostic equipment.

Since June 2013 laboratory “Research Training Center for Animal Disease Diagnostics” (RTCADD) at the Institute of Veterinary Medicine began to work at full capacity.

It acts under the Laws of Ukraine, current national and international standards, legal acts and other documents that ensure the quality of the results.

Laboratory RTCADD is accredited in accordance with the requirements of GOST ISO / IEC 17025: 2006 by Slovak National Accreditation Service (SNAS). This Standard establishes general requirements for the competence of laboratories in research / testing and calibration, including sampling, testing and calibration conducted in accordance with standard and non-standard methods and techniques developed laboratory.

The laboratory carries out effective diagnosis of infectious diseases of animals (cattle, pigs, horses, rabbits) and birds according to the “certificate of attestation №45-414 / 2013″ using classic and current methods of research. Resolution № 21–13 to work with agent II–IV pathogenicity groups issued by the Kyiv City Regime Commission 07/31/13.

To ensure an external laboratory quality control Centre actively participates in national and international rounds of the inter-laboratory comparison test. Activities of the laboratory periodically checked during internal audits. The results of the audits are used to develop and improve our operations.

Particular importance is attached to professional development, each year training of laboratory staff improving their skills during internships at leading research institutions of veterinary medicine in Europe and the US, participate and organize international and national scientific conferences and seminars.

Laboratory Staff:

Halka Ihor Vasyliovych- Head of the Laboratory, PhD of Vet Sci, Candidate of Veterinary Sciences (specialty 16.00.03 – veterinary microbiology, epizootology, infectious diseases and immunology). Phone: +38-050-546-40-47; E-mail: ptica2005@ukr.net

Muzykina Larysa Mykolaivna – Deputy Head of the Laboratory; Mobile tel. +38-063-639-35-45; E-mail: loramuzykina@i.ua

Sidorenko Tetyana Volodymyrivna, leading doctor of veterinary medicine;

Bondar Svitlana Mykolaivna, leading engineer;

Pogrebnyak Olga Petrivna, scientific researcher;

Chekhun Artem Ivanivich, scientific researcher;

Gnatenko Alona Vasylivna, junior scientific researcher;

Molozhanova Alona Vasylivna, leading doctor of veterinary medicine;

Mandygra Svitlana Stanislavivna, doctor of veterinary medicine;

Kovalenko Anna Andriivna, doctor of veterinary medicine;

Maksymov Dmytro Oleksandrovich, engineer;

Medvedcka Irina Mykolaivna, laboratory assistant;

Borovic Olena Yakivna, laboratory assistant.

Main Tasks of the Laboratory

• scientific and methodological support for the prevention of biological risks and threats concerning livestock;
• participation in the implementation of the “National Program of regulation of an adequate system of monitoring and biological risks evaluation”;
• improving the scientific and methodological level of fundamental and applied research;
• organization of management quality system of scientific support of samples research in accordance with international requirements;
• good laboratory practice for the diagnosis of animal diseases;
• development of the biosafety control system and management of biological risks;
• development of new quality control system and safety of biological preparations;
• increasing of implementation volume of modern biotechnologies in livestock production.

Main activity directions of the Laboratory

1. Scientific-research;
2. Diagnosis;
3. Education and training.

The research direction involves the participation in performance of scientific topics.

Independent molecular-genetic and serological diagnostic tests of animal infectious diseases are carried out using modern equipment at the Laboratory «RTCADD» as well as chemical-analytic tests.

PCR-diagnostics (PCR Real time):

• RNA detection of Porcine Epidemic Diarrhea virus;
• DNA detection of Chlamydia;
• DNA detection of Porcine Circovirus type II;
• RNA detection of Porcine Reproductive and Respiratory Syndrome virus (PRRS);
• DNA detection of Mycoplasmosis Swine and Birds pathogen;
• DNA detection of Bovine Infectious Rhinotracheitis virus;
• DNA detection of Equine Herpes virus Type 1;
• RNA detection of Rotavirus infection;
• DNA detection of Brucellosis pathogen;
• RNA detection of Bovine Viral Diarrhea virus and others.

ELISA - diagnostics (ELISA):

• Leucosis;
• Influenza A;
• Porcine Reproductive and Respiratory Syndrome (PRRS);
• Porcine Epidemic Diarrhea (PED);
• Mycoplasmosis in Swine;
• Aujeszky’s disease;
• Bovine Infectious Rhinotracheitis (BIR);
• Bovine Viral Diarrhea;
• Bovine Respiratory Syncytial Infection;
• Parainfluenza – 3;
• Porcine Circovirus type II;
• Haemophilus Polyserositis in Pigs;
• Actinobacillosis Pleuropneumonia in Pigs;
• Porcine parvovirus;
• Chlamydiosis and others.

Chemical-analytic tests:

1. Determination of the volume fraction of ethyl alcohol in the composition of aqueous solutions by Biochromate-iodometric method;
2. Determination of the mass fraction of iodine in the composition of aqueous and alcohol solutions by Iodometric method;
3. Determination of the mass fraction of chlorine in the composition of aqueous solutions by Iodometric method;
4. Determination of the mass fraction of formaldehyde in the composition of aqueous solutions Iodometric method;
5. Determination of the mass fraction of glutaraldehyde in the composition of aqueous solutions by Iodometric method.

Education and training activity of the laboratory includes lectures and practical classes concerning infectious diseases of animals by PCR and ELISA according to “Training programs for specialists of veterinary medicine regarding laboratory diagnosis”.

The laboratory conducts advanced training of specialists of veterinary medicine:

• lectures;
• methodicaland practicaltraining in virology;
• methodicaland practicaltraining inbacteriology;
• training in work rules with pathological material in BSL-2 facility as well as the rules of packaging and shipping of biological material;
• methodological and practical study of the equipment for PCR and ELISA diagnostics.
  

Main scientific achievements of the laboratory in 2013–2015.

In 2014 a certificate of accreditation according to the requirements of GOST ISO/IEC 17025:2006 from the Slovak National Accreditation Service (SNAS) has been received, which enables to carry out diagnostic tests and to publish results recognized in Europe and worldwide.

In 2015, according to the order of the State Veterinary and Phytosanitary Service of Ukraine №13 from 26.01.2015 to monitoring Porcine Epidemic Diarrhea (EDS) virus in the laboratory RTCADD was conducted research of biological material from pigs for the presence of RNA of EDS virus by PCR in real time.

International Cooperation:

1. Defense Threat ReductionAgency ofthe USA (DTRA)
2. National Veterinary Research Institute (Pulawy, Poland)
3. Republican Scientific-Research Subsidiary Unitary Enterprise “The S.N. Vyshelessky Institute of Experimental Veterinary Medicine” (Minsk, Republic of Belarus)
4. Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health.
5. Institute ofVeterinary Medicine, Mongolia
6. Republican State Enterprise National Reference Center for Veterinary Medicine, Republic of Kazakhstan

Cooperation in Ukraine

The center collaborates with veterinary research institutions and universities of such areas as:

• internships of teaching staffusing modern equipment;
• joint performance of laboratory andscientific researches;
• implementationofinternationalgrants;
• trainingoffutureresearch and teachingstaff and specialists of veterinary medicine;
• training of doctors of the State Veterinary Medicine Laboratories.

Agreements with leading educational institutions of Ukraine are concluded, including the National University of Life and Environmental Sciences of Ukraine, NSC “Institute of Biology” Kyiv National Taras Shevchenko University.

Rooms and equipment

Structure of the Laboratory:

1. Virologicsector;
2. Bacteriologicalsector;
3. Chemical Analysis Sector.

Room for serological tests

Chemical Analysis Sector