Research Training Center for Animal Disease Diagnostics

 

Head of the Laboratory Halka Ihor Vasyliovych, PhD of Vet Sci, Candidate of Veterinary Sciences

Head of the Laboratory Halka Ihor, PhD in Vet Sci

Halka Ihor  (specialty 16.00.03 – veterinary microbiology, epizootology, infectious diseases and immunology).

Phone: +38-050-546-40-47

E-mail: ptica2005@ukr.net

Muzykina Larysa – PhD in Veterinary Sciences, Head of the Virology Sector of the laboratory.
Tel .: (063) 639-35-45

To improve the level of service, to maintain constant partnership and respect for the best professional practices in the laboratory, please fill in the feedback form and send it to the E-mail: ptica2005@ukr.net

 Staff of laboratory

Staff of laboratory

Laboratory History

The center was built under the “Biological Threat Reduction Program” and US Agreement on cooperation in preventing the proliferation of technology, pathogens and expertise that could be used in the development of biological weapons. In October 2010 the State Committee of Veterinary Medicine of Ukraine, National Academy of Agrarian Sciences of Ukraine and the Department of Threat Reduction in Kyiv, and the United States Embassy in Ukraine signed a memorandum on technical assistance for the “Reduction of biological threat in Ukraine” project.

Institute of Veterinary Medicine received technical assistance in the form of established and financed by the American side of the Training center and teaching laboratory Biosafety Level BSL-2 with a conference hall. Refurbished and equipped 623 m2, installed ventilation system, generator backup power, sophisticated diagnostic equipment.

Since June 2013 laboratory “Research Training Center for Animal Disease Diagnostics” (RTCADD) at the Institute of Veterinary Medicine began to work at full capacity.

It acts under the Laws of Ukraine, current national and international standards, legal acts and other documents that ensure the quality of the results.

Laboratory RTCADD is accredited in accordance with the requirements of GOST ISO / IEC 17025: 2006 by Slovak National Accreditation Service (SNAS). This Standard establishes general requirements for the competence of laboratories in research / testing and calibration, including sampling, testing and calibration conducted in accordance with standard and non-standard methods and techniques developed laboratory.

The laboratory carries out effective diagnosis of infectious diseases of animals (cattle, pigs, horses, rabbits) and birds according to the “certificate of attestation №45-414 / 2013″ using classic and current methods of research. Resolution № 21–13 to work with agent II–IV pathogenicity groups issued by the Kyiv City Regime Commission 07/31/13.

To ensure an external laboratory quality control Centre actively participates in national and international rounds of the inter-laboratory comparison test. Activities of the laboratory periodically checked during internal audits. The results of the audits are used to develop and improve our operations.

Particular importance is attached to professional development, each year training of laboratory staff improving their skills during internships at leading research institutions of veterinary medicine in Europe and the US, participate and organize international and national scientific conferences and seminars.

Main Tasks of the Laboratory

  • scientific and methodological support for the prevention of biological risks and threats concerning livestock;
  • participation in the implementation of the “National Program of regulation of an adequate system of monitoring and biological risks evaluation”;
  • improving the scientific and methodological level of fundamental and applied research;
  • organization of management quality system of scientific support of samples research in accordance with international requirements;
  • good laboratory practice for the diagnosis of animal diseases;
  • development of the biosafety control system and management of biological risks;
  • development of new quality control system and safety of biological preparations;
  • increasing of implementation volume of modern biotechnologies in livestock production.

Main activity directions of the Laboratory

  1. Research;
  2. Diagnosis;
  3. Education and training.

The research direction includes performance of science topics.

Independent serological, molecular and genetic diagnostic tests of animal infectious diseases are carried out using modern equipment at the laboratory «RTCADD».

PCR-diagnostics (PCR Real time):

  • RNA detection of Porcine Epidemic Diarrhea virus;
  • RNA detection of Classical Swine Fever;
  • DNA detection of African Swine Fever;
  • DNA detection of Porcine Circovirus type II;
  • RNA detection of Porcine Reproductive and Respiratory Syndrome virus (PRRS);
  • DNA detection of Bovine Infectious Rhinotracheitis virus;
  • RNA detection of Bovine Viral Diarrhea virus;
  • RNA detection of Rotavirus infection;
  • DNA detection of Mycoplasmosis Swine and Birds pathogen;
  • DNA detection of Chlamydia;
  •  DNA detection of Brucellosis pathogen and others.

ELISA - diagnostics (ELISA):

  • Leucosis;
  • Influenza A virus;
  • Porcine Reproductive and Respiratory Syndrome (PRRS);
  • Porcine Epidemic Diarrhea (PED);
  • Mycoplasmosis in Swine;
  • Aujeszky’s disease;
  • Bovine Infectious Rhinotracheitis (BIR);
  • Bovine Viral Diarrhea;
  • Bovine Respiratory Syncytial Infection;
  • Parainfluenza – 3;
  • Porcine Circovirus type II;
  • Haemophilus Polyserositis in Pigs;
  • Actinobacillosis Pleuropneumonia in Pigs;
  • Porcine parvovirus;
  • Chlamydiosis and others.

Education and training activity of the laboratory includes lectures and practical classes concerning infectious diseases of animals by PCR and ELISA according to “Training programs for specialists of veterinary medicine regarding laboratory diagnosis”.

The laboratory conducts advanced training of specialists of veterinary medicine:

  • lectures;
  • methodical and practical training in virology;
  • methodical and practical training inbacteriology;
  • training under SOPs for pathological material usage in BSL-2 facility condition as well as the rules of packaging and shipping of biological material;
  • methodological and practical study of the equipment for PCR and ELISA diagnostics.

Main scientific achievements of the laboratory in 2013–2015

In 2014 a certificate of accreditation according to the requirements of GOST ISO/IEC 17025:2006 from the Slovak National Accreditation Service (SNAS) has been received, which enables to carry out diagnostic tests and to publish results recognized in Europe and worldwide.

In 2015, according to the order of the State Veterinary and Phytosanitary Service of Ukraine №13 from 26.01.2015 the laboratory RTCADD was authorized to conduct monitoring for Porcine Epidemic Diarrhea (EDS) virus.

International Cooperation:

The laboratory RTCADD collaborates with different international agencies and research institutes:

  1. Defense Threat ReductionAgency ofthe USA (DTRA);
  2. National Veterinary Research Institute (Pulawy, Poland);
  3. Republican Scientific-Research Subsidiary Unitary Enterprise “The S.N. Vyshelessky Institute of Experimental Veterinary Medicine” (Minsk, Republic of Belarus);
  4. Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health;
  5. Institute ofVeterinary Medicine, Mongolia;
  6. Republican State Enterprise National Reference Center for Veterinary Medicine, Republic of Kazakhstan.

Structure of the Laboratory:

  1. Department of Virology;
  2. Department of Bacteriology;
  3. Department of Chemical Analysis.