Laboratory of Myсotoxicology

Fields of research:

The main scientific direction of the laboratory is the study of spreading toxicogenic fungi-producing mycotoxins in feed and food.

In the laboratory mycotoxicology developed technology for producing the most common mycotoxin standards. Developed methods for identifying these mycotoxins using thin layer chromatography, carried out experiments on animal mycotoxicoses. Methods for determination of mycotoxins in animal feed. One of the reliable prevention of fodder stress and subclinical mycotoxicosis that cause significant economic damage to livestock and poultry – is to find means of reducing the negative impact of mycotoxins.

Laboratory works on State scientific subjects in 2014–2015 32.02.02.06 P “Study of phytosanitary condition of feed grains and animal feed.” Program 32. Scientific support program epizootic welfare, biosecurity, animal health and veterinary-sanitary quality of animal products (“Biological safety and animal health”). Subprogramme 2. Scientific and methodological support of monitoring, control methods and animal health measures on the quality and safety of animal products.

The main achievements of the laboratory

Allocated a considerable amount of different toxicogenic fungi-producing mycotoxins and studied the optimal parameters toxic appearance in these fungi. Methods for determining mycotoxins in feeds and methods for isolating them in pure form. Experimentally reproduced patulinotoxicosis, zearalenontoxicosis, sterigmatocistintoxicosis, ohratoxicosis (1984–1988). At the same time studied the sensitivity of animals to toxins, clinical, haematological and pathological changes in acute and chronic toxicity within. Installed LD50 toxins for animals in acute experiments; chronic maximal ineffective, subthreshold and threshold dose. Studied cumulative properties and reasonable maximum allowable levels (MRLs) toxins in feed for fattening pigs.

Developed a screening method for the simultaneous detection of aflatoxin B1 in feed, patulin, Sterigmatocystin, zearalenone, T-2 toxin and deoxynivalenol. Published “Guidelines for the sanitary and mycological evaluation and improvement of the quality of feed”.

Laboratory staff developed specifications for standards 11 mycotoxins and established their manufacturing to provide a variety of scientific and industrial laboratories, for use as substances witnesses during trials micotoxicological feed and food:

  • Zearalenone TU – 46. 15. 22 – 93
  • Patulin TU – 46. 15. 20 – 94
  • Sterigmatocystin TU – 46.15.28 – 94
  • T-2 toxin TU – 46.15.035 – 94
  • Aflatoxin B1 TU – 46.15. 061 – 95
  • Kojic acid TU – 46.15.060 – 95
  • Aflatoxin B2 TU – 46.15.299 – 98
  • Aflatoxin G1 TU – 46.15.298 – 98
  • Deoxynivalenol TU – 46.15.367 – 99
  • Ochratoxin A TU – 10.07.017 – 88
  • Citrinin TU – 10.07.019 – 88

Established and reasonable maximum allowable levels (MRLs) of T-2 toxin in feed for chickens, calves and cattle for fattening, which facilitates diagnosis, develop more targeted preventive measures and decide on the use of animal products and animal feed.

The substantiation of the maximum allowable levels:

  • Justification of the maximum allowable levels (MRLs) of patulin in feed for pigs for fattening (17.06.1985).
  • Justification of the maximum allowable levels (MRLs) of zearalenone in feed for pigs (09.10.1985).
  • Justification of the maximum allowable levels (MRLs) for pigs Sterigmatocystin group feeding (25.10.1990).
  • Justification of the maximum allowable levels (MRLs) Ochratoxin A in feed for pigs feeding groups (25.12.1990).
  • Temporary MPC T-2 toxin in feed and water (02.02.1990).
  • Justification of the maximum allowable levels (MRLs) of T-2 toxin in feed for calves and cattle for fattening (09.12.1997).
  • Justification of the maximum allowable levels (MRLs) of T-2 toxin in feed for chickens (09.12.1997).

The laboratory has developed a number of techniques:

  • Preparation procedure of cojic acid, the toxic metabolite of fungi (01.10.1991),
  • Method of determination of aflatoxin B1 in silage and haylage (22.03.1995),
  • Method of determining the miktoksinu Koev acid in silage and silage (02.1994),
  • Method of determination of zearalenone in silage and haylage (10.02.1994),
  • Method of determination of patulin in silage and haylage (02.08.1995),
  • The rules for determining mycotoxin patulin in feed and food (09.04.1996),
  • Terms of simultaneous detection of aflatoxin B1, patulin, Sterigmatocystin, T-2 toxin and zearalenone in different feeds (09.04.1996),
  • Method of determining the Sterigmatocystin in feed and food (10.06.1997),
  • The rules for determining aflatoxin B1 in feeds (10.06.1997),
  • The rules for determining zearalenone in feed (10.06.1997),
  • Method of determining the sorption activity of prevention of mycotoxicosis (21.12.2012),
  • Specifications feed additive (Vitakorm) TU – 15.7 – 3125325 – 001: 2011.

Lab work repeatedly represented at the State Prize of Ukraine in the field of science and technology. In 2005, the Presidium of the Academy of Agricultural Sciences said first prize laboratory staff and its achievements.