Laboratory of anthrax

Head of the laboratory – PhD Tarasov Oleksandr Anatoliiovich

Head of the laboratory – PhD Tarasov Oleksandr 

Tarasov O. graduated with honors from the Faculty of Veterinary Medicine of  National Agrarian University in 1996. He defended his thesis by the specialty 16.00.03 – veterinary microbiology, epizootology, infectious diseases and immunology. Awarded the title of senior researcher in 2010.

specialists of laboratory

specialists of laboratory

Brief  history

The laboratory of anthrax is one of the new created structural divisions of the Institute of Veterinary Medicine of NAAS, which was established in early 2012 on the basis of the laboratory of bacterial diseases. The last one laboratory was established in the first year of the Ukrainian Scientific-Research Veterinary Institute (1977). The head was an outstanding scientist in the field of veterinary medicine, Doctor of Veterinary Sciences, Professor, Academician of the NAAS of Ukraine Zaviriukha A.I.

His significant contribution to the study of anthrax in Ukraine is:

  • “Catalogue of anthrax hot spots on the territory of the Ukrainian Republic in the period 1920-1978″ Edited N.Y. Chabliy;
  • “Catalogue of anthrax burial grounds for the period 1920-1978 ” Edited N.Y. Chabliy;
  • Abacillar vaccine “Antrakol” for the treatment and prevention of anthrax farm animals;
  • Live vaccine against anthrax from strain K-79Z;
  • Anthrax antigen for reaction precipitation (Ascoli).

The main scientific directions:

  • study the spread of anthrax in Ukraine, investigation the state of anthrax burial grounds in different regions;
  • selection of the B. anthracis and creating new vaccine strains;
  • improvement of existing control and diagnosis means of anthrax;
  • development of the concept model of anthrax outbreaks risk prediction in Ukraine;
  • the study of theoretical and practical aspects of infectious disease of pigs;
  • the creation of specific preventive means against the most common bacterial infections of pigs.

The main scientific results in 2010-2015 years

As a result of research works it have been developed for the practice of veterinary medicine few immunobiological and chemical preparations:

  • emulsion vaccine against swine erysipelas, inactivated.
  • live dry vaccine against swine erysipelas. Technical conditions. DSTU 6079: 2009;
  • formolvaccine polyvalent aluminum hydroxide against ehsherihioza (colibacillosis) pigs from local strains of E. coli;
  • A set of sera for serological identification of strains of pasteurellosis;
  • vaccine against pasteurellosis, salmonellosis and colibacillosis pigs “Pasako”;
  • serum agglutination for the serologic identification of strains of E.coli serogroup B 157.
  • Biological products: DSTU 6079: 2009 “live dry vaccine against swine erysipelas,” “the emulsion vaccine against swine erysipelas inactivate» (№ 1933-04-0241-06 of 4 July 2006)
  • complex antibiotic drugs “Floksil- C” (registration certificate number 00459-01-08 AV / 1 of 20.07.2009 years), “Tilomitsin-B” (registration certificate number AB-00460-01-08 / 1 from 20.07. 2009), “Bioviton” (registration certificate № 00461-01-08 / 1 from 20.07.2009 years), a drug for disinfection in the presence of animals “Aerodez” (patent pending).

International collaboration :

National Veterinary Research Institute (Pulawy, Poland) – cooperation on anthrax diagnosis and survailance of the disease.

University of Florida, SEER lab – collaboration on the issues of B.anthracis ecological niche modeling, pathogen spreading and the development of strategies to control and eradication of anthrax.

Collaboration in Ukraine

  • State Service of Ukraine for Food Safety and Consumer Protection (Kyiv);
  • The Institute of Animal Biology (Lviv).

The main publications (2010-2015) :

  • Yanenko VM. Methods Vacillus anthracis spores extraction from different types of soil / VM Yanenko, p. M. Tereshchenko, NA Sheremet // Veterinary Biotechnology. – Is. 21. – 2012. – P. 143-148.
  • Yanenko VN Analysis methods for diagnosis of pasteurellosis of cattle and pigs / Yanenko VM, VM Yanenko // National Gazette vet.med Lviv National University. and biotechnology them. S.Z. Gzhytsky – T. 14. № 2 (52). h. 1. – Lviv. – 2012. – P. 397–403.
  • Nychyk SA-Definition Antilles zotstymnoyi activity B. anthracis cultures, B. mycoides, B cereus, B. subtilis / Nychyk SA,. Ayshpur A. Yes, Yanenko William M. Tereshchenko S., N. Sheremet // Veterinary Biotechnology O . //  Is. 21. – 2012. – P. 139–142.
  • Tarasov OA Study of antibiotic drugs fluorchinolone action groups in relation to the museum strains and isolates Erysipelothrix rhusiopathiae / Veterinary Biotechnology. – 2010. – №16 – P. 277-284.
  • Obrazhey AF , Tarasov OA Analysis of drugs for immunization of pigs erysipelas // Veterinary Medicine of Ukraine. – 2010. – №10. – P. 44-46
  • Dry Vaccine live pigs against erysipelas. Specifications. ISO 6079: 2009 / Obrazhey AF Sapeyko VP Tarasov OA, Zotsenko IA, Ostapets MG – Kyiv, State Committee of Ukraine, 2010.
  • Tarasov OA The study of antimicrobial substances and combinations of approaches to the study of new antibiotic drugs / Veterinary Biotechnology. – 2010. – №17 – P. 220-226.
  • Tarasov OA Study immunogenic properties strains Streptococcu suis, suitable for the manufacture of vaccines and testing methods to control immunogenicity / O.A.Tarasov, I.A.Zotsenko // Veterinary Biotechnology. – 2014. – №24. – P. 248-253.
  • Tarasov O.A. Investigation of antigenic affinity of Streptococcus suis isolates in  Ukraine / O.A. Tarasov, M.M. Babkina, S.M. Tereshchenko, I.A. Zotsenko, S.Yu. Krylenko // Veterinary biotechnology. – 2017. – № 30. – P. 224–228.
  • Babkina M.M. Determination of antibacterial properties of triazines in regards to Salmonella typhimurium / M.M. Babkina, O.V. Vasilchenko, O.M. Deryabin, A.A. Tarasov, A.M. Golovko, L.G. Palchikovskaya // Animal Biology. – 2017. – Vol. 19, № 1. – P. 16–23.
  • Hudz N. Analysis of Possible Anthrax Outbreaks in Ukraine Using Modern Approaches / N. Hudz, A. Tarasov, M. Babkina, S. Nychyk // ASM Microbe 2017,  June 1–5, 2017, New Orleans, LA. – New Orleans. – 2017.
  • Kracalik I.T. Rethinking livestock vaccination in Ukraine: modeling the environmental risk of wildlife and livestock anthrax in Ukraine to aid in police / Kracalik, Maksmy Bezymennyi, Oleksandr Tarasov, Natalia Hudz, Jason K. Blackburn // The International Conference on Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis» («Bacillus ACT»). – 2017. – Р. 87.
  • Tarasov O.A. Study of the synergy effect of benzalkonium chloride on antimicrobial activity of vegetable essential oils / O.A. Tarasov, V.P. Sapeyko, O.A. Aishpur, M. Babkin, S.M. Tereshchenko, I.A. Zotsenko // Veterinary biotechnology. – 2018. – №32 (2). – P. 529–537.
  • Babkina M.M. Investigation of the antibacterial action of modified heterocyclic compounds of substituted acridones against Staphylococcus aureus / M.M. Babkin, O.V. Vasilchenko, O.M. Deryabin, O.A. Tarasov, A.M. Golovko, L.G. Palchikovskaya // Animal biology .– 2018 – Vol. 20, Is. 1. – P. 9–15.
  • Ukhovskyi V.V. Using of glycerol and DMSO for Leptospira interrogans cryopreservation. / V.V. Ukhovskyi, P.P. Anatoliy, O.A. Tarasov, T.М. Ukhovska, A.P. Paliy // Probl Cryobiol Cryomed 2019 – 29(1) – 102–106. DOI: https://doi.org/10.15407/cryo29.01.102.

Patents :

  • Patent of Ukraine for useful model 10833. Ukraine, S12N1 IPC / 20 A61K39 / 02. The strain of bacteria Erysipelothrix rhusiopathiae M 2VK for production of inactivated vaccines against swine erysipelas / OA Tarasov AF Obrazhey, OM Deryabin (Ukraine) / Institute of Veterinary Medicine UAAS – № u200507495; Appl. 27.07.2005; Publish. 15.11.2005, byul.№ 11 – 6 p.
  • Patent for useful model №43460. Ukraine. IPC (2009) A61R1 / 00. Complex vitamins and probiotic drug Bioviton / Sapeyko SV Obrazhey AF Sapeyko VP Tarasov OA, Beard IL – Appl. 26.08.2008; Publish. 25.08.2009, Bull. №16.
  • Patent of Ukraine for useful model 127307 IPC A61K 8/92 (2006.01). Therapeutic disinfectant based on vegetable essential oils / O.A. Tarasov, V.P. Sapeyko, M.M. Babkin, N.V. Hudz, S.Yu. Krylenko. // Appl. 23.02.2018; Publish. 25.07.2018, Bull. №16.