HISTORY INSTITUTE

Ukraine; 03151,  Kieiv,  st. Donetsk, 30;

phone / fax: + 38-044-245-78-05;

E-mail:vet@ivm.kiev.ua  

Web site: www.ivm.kiev.ua

Institute of Veterinary Medicine of the National Academy of Agrarian Sciences of Ukraine (until May 4, 1992 called – Ukrainian Scientific Research Veterinary Institute ) was established in Kyiv in 1977 on the basis of the Ukrainian Research Institute of Experimental Veterinary Medicine (URIEVM) , which was led by Candidate of veterinary science G.F. Bondarenko from September 16, 1968) in accordance with of the USSR Cabinet of Ministers decree # 351 of 1977, July 4.

Corresponding member of the All-Union Academy of Agricultural Sciences and Ukrainian Academy of Agricultural Sciences, Honored Scientist of Ukraine, Professor, Doctor of Veterinary Sciences Anatoly Ivanovich Sobko was appointed Its first director and for more than 20 years was the head of Institute and managed virology laboratory (1977-1993) created by the order #47 as of 1977, April 14. Virology laboratory started its work earlier, as a division of the URIEVM branch.

The first head of the Virology Laboratory – Corresponding Member of the All-Union Academy of Agricultural Sciences and Ukrainian Academy of Agricultural Sciences, Professor, Doctor of Veterinary Science Sobko Anatoly Ivanovich.

Professor Anatoliy Sobko is the world famous scientist and was at the sources of such important problems of veterinary science as Infectious diseases of pigs, foot and mouth disease of farm animals, researches of viruses and bacteria associations. He devoted most of his life to the veterinary virology, forming and realization into veterinary practice of new ideas and developments, organization of science, its development and prosperity. Carried out by Anatoliy Sobko deep fundamental researches allowed to develop a set of clinical and laboratory methods and create more than 30 biological products for the diagnosis and prophylaxis of animal viral and bacterial diseases, most of researches were awarded by gold and silver medals of the All-Union National Economy Exhibition, international exhibitions and confirmed by patents. Anatoliy .Sobko has published more than 130 articles printed 5 monographs, received 50 invention certificates and patents. In Vietnam was issued a guide for pigs diseases edited by Anatoliy Sobko.

An order №1 issued of 1977, July 6 by Ukrainian Scientific-Research Veterinary Institute, signed by director Anatoliy Sobko, declares, that in accordance with the resolution of the USSR Cabinet of Ministers State Committee for science and technology the newly formed Institute, including laboratory of virology is tasked with conducting research in primary areas:

• development and implementation of measures for the protection of industrial livestock complexes and specialized farms from bringing infectious diseases;
• development of tools and methods for diagnosis of diseases in a condition of high animal concentration;
• development of animals prophylaxis of infectious animal diseases;
• development of new methods of treatment and preventive treatment of animals;
• production for need of Republic animal husbandry of small series of new specific means of combating infectious diseases.

The Institute has developed by the Regulations on the research activities of virology laboratory, its subordination, structure, composition of research staff, as well as the profile of the work and the main task.

The main direction of research of virology laboratory, according to the Regulations, is the study of infectious animal diseases of viral etiology, development of methods of diagnosis and means of active prophylaxis. Based on the primary task, the laboratory is engaged in the improvement of methods for the isolation and identification of viruses – animal disease-producing factors, the study of the morphology, physic-chemical and immunological properties of viruses – animal disease-producing factors, the study of the pathogenesis and immunogenesis of viral infections, the development of methods of industrial cultivation of viruses, which are used for the manufacture of diagnostics and vaccines, development schemes of the differential diagnosis of viral diseases of animals, the development of manufacturing technology, control and use of live virus vaccines, the creation of a museum of reference strains of viruses and epizootic – animal diseases, selection and preparation of production viruses strains for manufacturing diagnostic and vaccine preparations, the study the effectiveness of chemotherapeutic agents for the treatment and prophylaxis of animal viral diseases, the study of the epizootic situation, forecasting and developing of preventive measures on infectious animal diseases.

In the laboratory were operated mobile group that traveled to complex infectious disease outbreaks in livestock enterprises through the entire Soviet Union (d.v.s., prof.,Sobko A.I, d.v.s. Stancheus A.P., d.v.s. Priskoka V.A., c.v.s. Kucheryavenko O.O.,c.b.s. Kucheryavenko A.A., k.v.n.,Vabishchevich F.S., Khomenko V., Makarenko A.V., Manziy K., Matuzenko M. and others). Thanks to their travels the Institute was replenished by the collection of pathogenic viruses, which according to developed technologies further served as basis for the creation of vaccines. Designed and manufactured by Institute’s employees vaccines due to efforts of the mobile teams were implemented with success in veterinary practice, testing of new schemes of animal viral infections prevention and eradication.

The laboratory performs a wide range of works on microbiological, biochemical, serological, diagnostic studies in all cases of suspected viral infections of animal disease; provides consulting service on the use of laboratory results in the veterinary practice.

After Anatoliy Sobko this division for 35 years period was led by:

• Candidate of veterinary sciences Vabishchevich F,S. (17.05.1993 – 05.05.1997)
• Doctor of Veterinary Sciences, Professor Starcheus A. P (6.17.97 – 1.06.2004)
• Doctor of Veterinary Sciences Sinitsyn V.A. (1.06.2004- 31.12 .2010 g),
• Doctor of Veterinary Sciences Klestova Z.S. (With 1.01.2011 –till the.present).

During the period of Virology laboratory existence, by its division, were created separate researches units of the Institute – Laboratory of diagnostics (1980.), Laboratory of cell culture technology cultivation of viruses, Laboratory for research on the technology for production of inactivated vaccine against classical swine fever (1988) and others.

Candidate of veterinary sciences   Alexander Kucheryavenko served as head of the Virology laboratory during period 1980 to 1984. From 1984 till 1986 served as a head of this laboratory candidate of biological sciences Alexey Kucheryavenko.

Besides in the Virology laboratory were established, operated and performed scientific tasks subsequent sectors:

• sector of electron microscopy;
• sector for the study of pigs transmissible gastroenteritis (TGP);
• sector for the study of genetic resistance of animals to infectious diseases;
• sector for the study of pigs enteroviral gastroenteritis ;
• sector pathology and therapy of infectious diseases;
• sector of gnotobiology;
• sector of interferon.

Sector electron microscopy under the direction of candidate of medical sciences Kupchynskyi L. (11/16/77 – 10/01/97)

The main areas: the development of rapid identification of viruses, including animals coronavirus, enteroviruses, rotavirus for rapid diagnosis of pigs viral gastroenteritis, animal influenza, caliciviruses, others and the development of prophylaxis methods and combating them.

Research was conducted to find ways of cultivation and concentrating of viruses, identifying of bacterial and viral interactions and others.

Sector for the study of pigs enteroviral gastroenteritis under guidence of candidate of medical sciences Tatska V. N. (since 1993)

The main directions: etiology study of pigs diarrheal diseases, isolation and identification of viruses – pathogens of pigs enteroviral gastroenteritis, the development of means of specific prophylaxis and combating pigs enterovirus gastroenteritis. The study of enteroviruses that cause abnormalities of pigs reproductive function – the so-called SMEDI syndrome. Development of a “kit for the diagnosis of pigs enteroviral diseases with SMEDI syndrome” and vaccines against pigs enteroviral gastroenteritis.

Sector pathology and therapy of infectious diseases under the supervision of the candidate of medical sciences V.G. Kvachov (since 1993).

Main areas: the creation and improvement of the methodological base for the study of cellular immunity in pigs and other animals, the study of progenitor cells in immunocompetent system, as well as the creation of tests for their integrated assessment of transformation and functional characteristics. Investigation of the mononuclear phagocyte system and their hematogenic predecessors as advanced level of defense against pathogens, recognition and induction of immune responses.

Sector for the study of the genetic resistance of animals to infectious diseases under the supervision of the candidate of biological sciences Z.S. Klestova (from 07.01.1993 g ..)

Main research areas: promoting preservation of health and gene pool of productive animals of Ukraine in the aftermath of man-made catastrophe (Chernobyl), carrying out diagnosis and prophylaxis of immunological and cytogenetic damage caused by infectious agents and radionuclides (including field pathogenic strains of microorganisms), a comparative assessment of genetic resistance of existing breeds and lines of farm animals and birds to infectious diseases, development of protective and antimutagenic agents, development of new methods of genetics and biotechnology, identifying the impact of prophylactic agents (including live and inactivated vaccines) on the state of animals genome for the development of safe animal cell genome prophylaxes drugs used in veterinary practice.

Sector for the Study of transmissible gastroenteritis of pigs (CBC) under the direction of the candidate veterinary sciences F.S.Vabischevicha (from 02.01.1991 g.)

Main research areas: isolation and identification of microorganisms, including viruses from pathological materials of sick animals; development, testing, manufacturing and introduction of immunobiological preparations; study the physicochemical properties of coronavirus strains of pigs; production of vaccines against the CBC.

Gnotobiologii sector under the guidance of doctor of veterinary sciences Volynets LK and senior researcher Makarenko A.V. (9.09.91-15.05.95)

Main areas: organization of tehnology of obtaining and growing gnotobiotiv animals (piglets gnotobiotiv) free from pathogenic microorganisms (BPA), manufacture and control of gnotobiological equipment, determining the status of BPA in pigs, to explore ways of feeding gnotobiotiv piglets and BPA in different periods of cultivation that allowed enough to significantly raise the level of methodological and validity of research in the study of disease in pigs.

Interferon sector-led AJ Karas (Z.11.05.92)

Main areas: the creation of a series of interferon preparations and prophylactic agents against infectious diseases of animals and birds on the basis of transfer factor (interferon splenotsitarny for pigs and cattle, aeronafek, pegnafek – drug detoxification and immunostimulatory effects, and others.).

Laboratory cell cultures under the supervision of candidate of veterinary sciences Subaev G.H., candidate of biological sciences Kucheryavenko Alexei Alexandrovich (…)

Main areas: acquisition and cultivation of primary cell cultures from different tissues (organs) of animal origin that are sensitive to the swine viruses, and the maintenance of continuous cell cultures, the use of which in the laboratory of virology allowed for the successful work on the isolation, identification of viruses, diagnosis and prevention of viral infections including the manufacture of vaccines and diagnostic products.

Laboratory for the study of technology for production of inactivated vaccine against classical swine fever under the leadership of doctor of veterinary sciences Priskoka V.A. started its activities in 1988.

The main areas: study of the biological properties of classical swine fever virus different strains; selection of the best strains for producing VKCHS inactivated vaccine; development of the method of cultivation of animal cells sensitive to classical swine fever virus; development of technology of inactivated vaccines against CSF virus from the culture and test its immunogenic properties in animals; to test inactivated vaccine on highly pathogenic strain VKCHS “Chi Burbot” develop normative and technical documentation for production of inactivated vaccines against CSF. It was created inactivated vaccine against classical swine fever for the developed technology.

Ensuring sustainable livestock veterinary prosperity largely depends on early diagnosis of diseases through the use of new modern methods of diagnosis. And so, in 1980, at the Laboratory of Virology was established a separate unit – laboratory of diagnostics. Head of the Laboratory was appointed Doctor of Veterinary Science A.P.Starcheus (1980 – 2004)

One of the topical areas of laboratory diagnosis was: diagnosis of viral diseases of pigs by known methods and development of new methods of isolation and identification of pathogens, the development of products for the diagnosis of viral diseases of pigs.

Laboratory diagnosis employees continuously conducted the epizootic survey swine breed, studied massive retrospective serologic examinations, examined clinic disease bioassay was performed, studied pathological changes in bodies of dead animals of different age groups which were isolated more than 50 infectious diseases. Studying biological and physico-chemical properties of the isolates serological and electron microscopic their identification revealed that the etiological agents of gastroenteritis in pigs in the specialized farms and complexes are korona-, entero- and rotavirus. Was established special role entero- and rotavirus, which caused a massive disease associations and death of newborn piglets. In gnotobiotic piglets was studied complex pathological changes in rotavirus disease of pigs, which characterized as catarrhal-desquamative enteritis. Given the complex, often associated etiology of gastroenteritis of pigs similarity of clinical manifestations and pathological changes were developed and improved methods of laboratory diagnosis and differentiation of diarrheal diseases of pigs. Following extensive work on the study of the pathogenesis, development and introduction of new and more effective methods for diagnosis of transmissible gastroenteritis of pigs and enterovirus (TGS EVHS), rotavirus and parvovirus infection of pigs. As a result of the research, isolated and identified viruses causing widespread disease of pigs diarrhea developed new methods and tools for diagnosis and TGS EVHS -reaction neutralization, complement fixation test for the diagnosis and EVHS diagnostic kit for the diagnosis of pigs parvovirus infection.

Laboratory’s developments were completed by the necessary specifications and technical documentation for 5 diagnostic kits (specifications, guidelines for the manufacture and control guidelines for the application) that are approved and implemented in industry to detect transmissible, entero- and rotavirus gastroenteritis and pigs parvovirus disease

In 1990 at the laboratory diagnosis was established ELISA sector under the supervision of candidate of veterinary sciences Sinitsin V.A.

The program of the sof ector: development of rapid methods iahnostyky infectious diseases of farm animals on the basis of enzyme immunoassay (EIA); development of methods for chromatographic analysis to identify pathogens, purification and concentration of antigens used in the formulation ELISA; receiving immune serum diagnostic and fractionation of immunoglobulins, albumin, making immunoperoxidase conjugates. The technology for the production of diagnostic products ELISA method with the classical swine plague, disease Aueski, TGS entero- and rotavirus disease, Teschen disease, trichinosis swine salmonellosis. A well organized industrial production of diagnostic kits for ELISA. Developed high-tech method of purification and concentration of virus received and highly specific serum of animals suitable for setting reaction ELISA, immunoperoxidase received active, antyenterovirusni and antysvynyachi con “yuhaty developed solid phase ELISA method for detection of specific antibodies to the virus enzootic bovine encephalomyelitis virus disease and swine AUESKI developed technology of concentrated purified antigens and pathogens erysipelas and swine trichinosis, a method of obtaining highly purified chromatographic albumin from bovine blood serum for ELISA reactions developed ELISA method for in vivo diagnosis of trichinosis swine, worked parameters setting reaction and test method conducted in a production environment.

For the first time in Ukraine using ELISA diagnostic tests conducted extensive pathological material from infected animals. This stanovlena high sensitivity and specificity of the test systems and correlation ELISA results with existing laboratory methods. The first time the fundamental possibility of using ELISA method to study stress indicators immunity perfected level of specific antibodies in the blood serum of pigs vaccinated different vaccines against classical swine fever, swine erysipelas, followed by infection control.

The sector was accomplished scientific objectives

1991-1995 years:

• “Develop methods for diagnostic analysis of bacterial and viral infections, improve manufacturing enzyme immunoassay diagnostics based on receipt of chromatographically purified biological products”;
  In the laboratory of virology created and implemented in veterinary practice transmissible vaccine, rotavirus, enterovirus (caused 6.7, 8 enterovirus serotypes pigs) gastroenteritis of pigs.

Vaccines have been developed based on a selected pig farms during mass outbreaks of gastroenteritis virus isolates, of which after studying their physical and chemical properties, having attenuation process in cell culture, began to meet the requirements vaccine strains. Thus, in 1977 strain was isolated Univ, assigned to the 6th serogroup enteroviruses pigs classification Derbyshire J. (1982), in 1979 r.- strain “386/79″ – 8th serogroup later “P15 / 91 “- 7th serogroup. Also in the Laboratory of Virology k.v.n. Vabishevychem VS was isolated from diseased pigs transmissible gastroenteritis virus strain “P 1439/81.” All designed, created and worked in the laboratory of virology vaccine in pig farms have been widely tested and mass production implemented in practice, allowing prevent viral infections and receive significant economic benefit by providing state save money. In addition, the Virology laboratory staff is Co-CSF vaccine, parvovirusnoyi disease and others.

In 2004 in the IBM Academy of Agrarian Sciences, as a result of the reorganization, merger happened two laboratories (diagnostic and virology) was formed and thus a combined laboratory and diagnostic virology led by Doctor of Veterinary Science Sinitsina VA The purpose of research were fundamental research on regularities of respiratory disease of horses, and the establishment of scientific and theoretical approaches to their diagnosis on the basis of modern biotechnological methods. A serological monitoring and epizootological research in Ukraine on equine influenza viral arteritis of horses (LHC) and rhinopneumonia horses. Individuals have cell culture for virus reproduction arteritis of horses rhinopneumonia and optimized parameters of their cultivation. Envisage a PCR method for the diagnosis rhinopneumonia horses and WAC. Manufactured laboratory sample set for the diagnosis of HAC and rhinopneumonia horses micromethod a neutralization reaction. The method of ELISA for serological diagnosis of equine influenza.

Research in the laboratory of virology and diagnostics on respiratory disease in horses performed ELISA, RTGA, RDP, RN macro and micromethod in cell culture, PCR.

Implementation of State Research Projects
For public research tasks and topics approved Agrarian Sciences and the Ministry of Agriculture of Ukraine, virology laboratory conducted research and development, including executed:

in perіod 1977-1980 years:

• “Improve existing and develop new methods and tools for effective control of infectious diseases of pigs” (1976-1980 pp. – № 79005144);
• “Create a vaccine and develop technology enteric immunization of pigs against gastrointestinal diseases in pig industrial complexes of the Ukrainian SSR” (1979-1983 pp.) – №9067813
• “Development of methods and means of prevention of gastrointestinal diseases in pigs (colibacillosis and transmissible gastroenteritis”);
• “Develop diagnostic methods and means of preventing disease of pigs caused by associations of bacteria and viruses in the environment complexes. Submit to the Ministry of Agriculture of the USSR guidelines for their use and production schedules for the production of diagnostic kits and vaccines “;
• “Examine the edge Epizootology FMD improved measures of prevention and to develop predictive methods for the disease in the Ukrainian SSR.”

in the period 1980 – 1987 years:

• “Work out associated vaccine against korona- and enterovirus pigs gastroenteritis and determine scheme of its application”
• “Search virus vaccine strains (transmissible) gastroenteritis of pigs to develop means of specific prevention of disease” (1981-1983) – №0182. 9012427.
• “Prepare vaccine strain эnterovyrusa pigs and study its biochemical and physicochemical properties “;
• “Investigate pathogenesis,develop and introduce effective diagnostic methods pigs virus (transmissive) gastroenteritis and propose to USSR Ministry of agriculture appropriate recommendations”;
• “Work out and introduce advanced vaccine against pigs transmissive gastroenteritis “;
• “Create associated vaccine against pigs korona- enteroviral gastroenteritis, and develop a scheme of its application” (1983-1987).

in the period 1987 – 1992 years:

• “Explore epizootology of most common pigs infectious diseases and develop modern methods of diagnosis, prophylaxes and treatment”;
• “To study the paraimmunity mechanism and develop tools and methods immunic stimulation to improve prophylaxis and treatment of the most important farm animals diseases “;
• “Create and master the production of vaccine sets against transmissible gastroenteritis and rotavirus infection of pigs” (1986 -1992) – № 0187.0 076 236;
• “Work out and introduce inactivated vaccine against swine viral transmissible gastroenteritis. Produce and test pilot batch parcel of vaccine and give initial requirements for designing of industrial production. Produce first batch of vaccine in conditions of industrial production “(1986-1990) – № 0187.0 076 235;
• “Produce and test pilot batch parcel of vaccines set and give initial requirements for designing of industrial production. Produce first batch of vaccines set in conditions of industrial production “(1987-1990) – № 0187.0 076 236.
• “Develop and implement the technology to produce inactivated vaccine against classical swine fever and perspectives of its use in specific prophylaxis”) (1988-1992) – №UA01011486R;
• “Study distribution in svynovodcheskyh economyof and viral gastroenteritis and group SMEDY viruses among swine-breeding economis of Ukrainian SSR using ELISA based on ICA and other laboratory methods;
• “Give recommendations on the rational use of means of specific prophylaxis;
• “Examine etiological structure of pigs pnevmoenteritis in Ukraine farms and develop methods for diagnosis and integrated system of prophylaxis actions”;
• “Develop methods for diagnostic analysis of bacterial and viral infections, improve manufacturing enzyme of immunoassay diagnostics based on chromatographically obtained purified biologics”

in the period 1993 – 2000 years:

• “Diagnosis, correction and prevention of structural damage to the genetic apparatus of cells of animals caused by infectious, technological and environmental factors”;
• “To study the effect of pathogens on the genetic apparatus animal cells and to develop methods for correcting its damage caused by infectious, technological and environmental factors.”
• “To study the mechanism of cellular immune animals, ground staff and offer immunomodulating drug directional.”
• “To study the taxonomy of enteroviruses and develop a system of measures for diagnosis and prevention of enterovirus diseases of pigs.”
• “Develop manufacturing technology, control and use of enterovirus vaccine serogroup 7 pigs and explore its effectiveness in terms of production.”
• “To study the mechanisms of stimulation paraimunnyh and immune responses and develop recipes and technology of new therapeutic and preventive agents with immunomodulatory effects dlyapatohenetychnoyi treatment of viral and other gastrointestinal diseases of pigs.”
• “To prepare laboratory specimens and napivvyrobnychi polyvalent (crown-rotavirus) vaccine and test its effectiveness in terms eblahopoluchnyh farms.”
• “Prepare a series of research and study of rotavirus vaccine immunogenic activity and pigs per sow.”
• “Create a system diagnostic and prevention korona-, entero- and parvovirusnyh infections and SMEDI pigs.”

Laboratory of Virology and scientific diagnosis made public tasks:

In the period 2001 – 2005 years:

• “Develop and implement methods and tools for diagnosis, prevention of respiratory diseases of horses”;
• “To study the distribution of pig farms in Ukraine korona-, entero-, rotavirus using ELISA method and other methods, to give advice on the rational use of drugs specific prevention”;
• “Develop and implement diagnostic and prevention of gastroenteritis in pigs viral etiology.”

in the period 2006 -2011 years:

• “To develop new and improved methods of diagnosis of viral arteritis and equine influenza”;
• “To study the regularities of respiratory disease of horses and develop scientific and theoretical approaches to their diagnosis on the basis of modern biotechnological methods.”

In 2011, as a result of the reorganization of structural units of the Institute of Virology Division was created, which is the head doctor of veterinary sciences Zinaida S.Klestova.

Department of Virology included three laboratories – laboratory viral disease of horses – Head Doctor of Veterinary Science Sinitsyn Vitaly A.; laboratory viral disease of pigs (by 2011 -laboratoriya epizootology) – Head, Doctor of Veterinary Science Sytyuk Nikolai laboratory and antiviral drugs – Head Doctor of Veterinary Sciences, Zinaida S. Klestova.

Laboratory epizootology that in 2011 became part of the Department of Virology laboratory as viral diseases of pigs was organized September 15, 1968. Its first director was the candidate of veterinary sciences H.F.Bondarenko – known scientific and practical community as a specialist in foot and mouth disease and bovine leukemia.

In the following years the laboratory led epizootology: – Candidate of Veterinary Science Divinity G.K. (February 1975 – September 1985), who independently written and co-authored the following books: “The disease control measures,” 1987. “Konskyy large horned livestock Gadfly”, “Brucellosis” with PM Zhovanikom 1975, “Beshenstvo wild animals” with k.v.n. LP Hryshok and k.v.n. AY Sinitsyn, 1981, described the five diseases in the “Handbook on pig disease,” 1981.

Research Laboratory was directed to study epizootiology of rabies and foot and mouth disease and improving prevention and combat these infections and the development of these diseases example. Under the direction k.v.n. GK Bozhko laboratory scientists k.v.n. LP Hryshok, k.v.n. VI Polulyakh, k.v.n. VP Ermakov, k.v.n. MS The reason was studied epizootological features of foot and mouth disease, rabies in Ukraine, vaccine effectiveness in these infections carried cartography 3942 outbreaks of rabies reported in Ukraine during the 1980-1984 biennium. Principles of Forecasting features epizootic rabies, foot and mouth disease and vaccination recommendations and their elimination .

The next head of the laboratory was epizootology candidate veterinary sciences Shikov A.T. (September 1985r.- 2011).

During this period the complicated epizootic situation in Ukraine on CSF and AIN subject line laboratory was designed to study the epizootiology of classical swine fever and improvement activities to prevent and control the disease. Was developed and published disadvantaged inventory items registered in Ukraine in 1961-2004 gg., Containing the address of 775 points from disadvantaged CSF with supporting information, the method of diagnosis, the number of animals that are sick, died and reluctantly slaughtered, the source of pathogen and diseases which occur in vaccinated or unvaccinated livestock “her method of recovery. Materials are systematized by territorial and temporal basis. Prospects for further combat CSF 15.12.2000 p. Epizootology from the laboratory of the Institute of Veterinary Medicine of the Presidium of the Academies and the State Department of Veterinary Medicine of Ukraine Order of 15.12.2000 p. Created “Research and Diagnostic Center of classical swine fever.”

The main activities of the Centre have been identified:

• advising on monitoring, diagnosis and prevention of CSF;
• study the epizootic situation, forecast and development of preventive measures CSF;
• systematic examination and laboratory tests herd of pigs promkompleksiv, tribal, and other specialized farms, wild pigs on CSF;
• epizootic clinical examination, laboratory testing of blood serum and material in all cases of suspected disease CSF, the development of measures to eliminate possible outbreaks.

The laboratory epizootology IBM NAASU established work of Diagnostic studies of pathological material for CSF, which came from different regions of Ukraine from domestic and wild pigs using the methods of the MFA. ELISA and immunoperoxidase method.

The efficiency of the method of oral immunization of foxes against rabies and recommendations for prevention, combat rabies wildlife.

Studied tension postvaccinal immunity against classical swine fever, disease Aueski, erysipelas industrial complexes.

Testing immunogenicity of three vaccine CSF developed regulations for their use in farms of different categories.

Designed instructions for prevention and control of classical swine fever, rabies and guidelines for the use of vaccines against these diseases

Source material accumulated in the laboratory, defended 5 PhD theses.

Laboratory performed epizootology state scientific objectives:

in the period 1977-2000 was:

• «Develop a system for monitoring, diagnosis, prognosis and prevention of classical swine fever” (2000);
• «Explore epizootologoiyu classical swine fever and to develop an inventory of disadvantaged settlements of the disease on the territory of Ukraine for the period (1981-1998 gg.)”;
• «Develop and issue the USSR Ministry of Agriculture of the short- and long-term forecasts for FMD and a program to combat the infection of 12 five-year plan” (1986);
• «Develop a long-term forecast epizootic situation and program animal rabies control for 1986-1990.”;
• «To develop methods for prevention of rabies in wild animals, a comparative evaluation of the effectiveness of parenteral and enteral methods of immunization of foxes against rabies vaccine,” the USSR Ministry of Agriculture to issue appropriate recommendations (1983);
• «To study the boundary epizootiology of rabies, the effectiveness of vaccination in this disease and to develop methods of forecasting in the territory of the Ukrainian SSR” (1980);
• «To study the boundary Epizootology FMD measures to improve the prediction of the disease in the Ukrainian SSR” (1980);
• «To develop activities to create sustainable well-being in the country for FMD”
• «the reason for the occurrence and characteristics of foot and mouth disease in different regions of the country” (1977.).

Development proposals epizootology laboratory practice:

• «Recommendations for the prevention and elimination of FMD disease of pigs on pig farms and other farms producing pork”, approved by the Head Office of the Ministry of Agriculture veterinary 5.111984g USSR. №116-7;
• «Forecast for FMD epizootic situation in the Ukrainian SSR and recommendations for vaccine preventable to 1984″ approved by 08.03.1983, the Ministry of Agriculture of the USSR BS;
• «Forecast for FMD epizootic situation in the Ukrainian SSR and recommendations for vaccine preventable to 1985″ approved by 05.09.1984, the Ministry of Agriculture of the USSR BS;
• «Long-term prognosis for FMD epizootic situation in the Ukrainian SSR in the twelfth five-year plan and recommendations for vaccine preventable,” approved 19.12.1985g. BS USSR Ministry of Agriculture;
• «A device for fixation of small laboratory animals, and the method of its implementation” is the rationalization proposals from 10.01.1986g;

To promote knowledge of FMD and measures to combat it issued for mass circulation poster “FMD” (Kiev: Vintage, 1986).

Since November 2010, the laboratory epizootology ICM headed by the candidate of veterinary sciences Sityuk MP The main direction of scientific research laboratory was to develop a comprehensive system for monitoring, control and prevention of especially dangerous diseases of pigs on the territory of Ukraine.

Currently, the Department of Virology ICM NAAS of Ukraine conducted a study on government contracts for three research objectives:

for the period 2011 – 2015 were as follows:

• «To study the immunobiological properties and ecology of pathogens of viral respiratory diseases of horses, to develop modern means of diagnosis and prevention” (2011 – 2015)
• «To study the mechanisms of antiviral activity in derivatives of porphyrins, indoles, isonicotinic acid, nucleosides and their combinations” (2011-2015);
• «Develop a comprehensive system for monitoring, control and prevention of especially dangerous diseases of pigs on the territory of Ukraine” sub-step “Monitoring of wild boars relatively common viral disease of pigs” (2011 – 2013).

Preparation of highly qualified scientific personnel

In the department of virology at the INM 1.12.2011 year (for the period of 1977) were prepared and defended 4 doctoral and 19 master’s theses:

Doctor of Sciences Dissertations:

• Anatoly Pavlovich Starcheus – “Laboratory diagnosis of viral gastroenteritis of pigs” (16.00.03 – veterinary microbiology, virology, mycology and epizootology “) (1989). Scientific adviser: Doctor of Veterinary Sciences, corresponding member of the Academy of Agricultural Sciences and UAAN, Professor Anatoly Sobko.
• Viktor Yushchenko Priskoka – “Rotavirus and mixed ifektsii pigs” (16.00.03 – veterinary microbiology, virology, mycology and epizootology “) (1989). Scientific adviser: Doctor of Veterinary Sciences, corresponding member of the Academy of Agricultural Sciences and UAAN, Professor Anatoly Sobko.
• Vitaly A. Sinitsin – “Development and production of diagnostic kits for immunoassay with classical swine fever, Aujeszky’s disease, Teschen, erysipelas, leukemia cattle, swine trichinosis and their practical application (theoretical rationale, implementation)” (16.00.03 – Veterinary Microbiology and virology) (2003). Scientific Adviser – Doctor of Veterinary Sciences, Academician of UAAS, Professor Anatoly snowstorm.
• Zinaida S. Klestova – “Theoretical and experimental study of the genome control cells during viral infections in animals and the development of means of specific prophylaxis” (16.00.03 – veterinary microbiology and virology) (2006). Scientific consultants – Doctor of Veterinary Sciences, Professor, Corresponding Member of the Academy of Agricultural Sciences and UAAN Sobko Anatoly Ivanovich, doctor of veterinary sciences, professor, academician UAAN Fuchs Pauline Pavlovna..

Candidate of Sciences Dissertations:

• Suba GH – Distribution and clinical characteristics Epizootologichesky bovine leukemia in the Ukrainian SSR “(16.00.03 – veterinary microbiology, virology, epizootiology, Mycology) (1969). Supervisor – Doctor of Veterinary Science Korotych Alex Stepanovich.
• Valentina Polulyakh – “Materials study epizootic of FMD in the Ukraine during the period 1952 – 1973 years” (16.00.03 – veterinary microbiology, virology, epizootiology, Mycology) (1978). Supervisor – Doctor of Veterinary Sciences Evgeny V. Andreev and the candidate veterinary sciences Gregory F. Bondarenko.
• Ludmila Pavlovna Grishok – “Epizootologichesky characterization of rabies in the Ukrainian SSR” (16.00.03 – veterinary microbiology, virology, epizootiology, Mycology) (1978). Supervisor – Doctor of Veterinary Science Korotych Alex Stepanovich.
• Umar Alfaruh Idriss – “Identification of viruses isolated from patients with gastroenteritis of pigs” (16.00.03 – veterinary microbiology, virology, mycology and epizootology) (1982). Supervisor – Corresponding Member of the Academy of Agricultural Sciences and UAAS, Doctor of Veterinary Science, Professor Anatoly Sobko.
• Fyodor Seraphimovich Vabishevich – “Isolation and identification of viruses causing massive diarrhea in pigs in specialized farms” (16.00.03 – veterinary microbiology, virology, mycology and epizootology) (1986). Scientific adviser corresponding member of the Academy of Agricultural Sciences and UAAS, Doctor of Veterinary Science, Professor Anatoly Sobko.
• Oksana Fedorovna Blotskaya – “The reaction for the diagnosis of enteroviral RTZK gastroenteritis of pigs” (16.00.03 – veterinary microbiology, virology, epizootiology, Mycology and Immunology) (1988). Supervisor – Corresponding Member of the Academy of Agricultural Sciences and UAAS, Doctor of Veterinary Science, Professor Anatoly Sobko. – Olga E. Krasnobaeva – “parvovirus infection in swine farms in Ukraine” (16.00.03 – veterinary microbiology, virology, epizootiology, Mycology and Immunology) (1989). supervisor – Doctor of Veterinary Science Starcheus Anatoly Pavlovich.
• Nikolai Stepanovich reason – the “Group immunity and effectiveness of anti-epizootic measures during FMD” (16.00.03 – veterinary microbiology, virology, mycology and epizootology) (1989). Supervisor – Doctor of Biological Sciences, Doctor of Veterinary Sciences, professor, corresponding member of the Academy of Agricultural Sciences Onufriev VP
• Vitaly A. Sinitsin – “ELISA with the transmission gastroenteritis of pigs” (16.00.03 – veterinary microbiology, virology, epizootiology, Mycology and Immunology) (1989). Supervisor – Corresponding Member of the Academy of Agricultural Sciences and UAAS, Doctor of Veterinary Science, Professor Anatoly Sobko.
• Zinaida S. Klestova – “Biological properties of Vector-borne gastroenteritis you fair-haired pigs and its effect on the chromosome of animal cells” (16.00.03 – veterinary microbiology, virology, epizootiology, Mycology and Immunology) (1990). Scientific adviser, corresponding member of the Academy of Agricultural Sciences and UAAS, Doctor of Veterinary Science, Professor Anatoly Sobko.
• Boulanger Limonchy Andres Avelino – “solid state immunoelectron microscopy to identify you blond transmissible gastroeeterita pigs” (16.00.03 – veterinary microbiology, virology, epizootiology, Mycology and Immunology) (1992). Supervisor – Corresponding Member of the Academy of Agricultural Sciences and UAAS, Doctor of Veterinary Science, Professor Anatoly Sobko and PhD Alexeenko Ivan Prokofievich.
• Valentina P. Ostapenko – “Post-vaccination immunity against enteroviral gastroenteritis in pigs of all ages” (16.00.03 -veterinarna microbiology, virology, epizootiology, Mycology and Immunology “) (1995). Supervisor: Corresponding Member of the Academy of Agricultural Sciences and UAAS, Doctor of Veterinary Science, Professor Anatoly Sobko and PhD, Senior Researcher Tatska Valentine Nikiforovna.
• Vasily V. Gusakov – “Improving the laboratory diagnosis of equine influenza in hemagglutination inhibition (HAI) (16.00.03 – eterinarna microbiology and virology) (2000). Supervisor: Doctor of Veterinary Science, Professor Anatoly Pavlovich Starcheus.
• Ruslan A. Holubets – “Changes in the metabolic profile and blood lymphocyte DNA of individual sections of cattle in leukemia” (03.00.04 – biochemistry) (2003) Supervisor: Ph.D., Senior Research Fellow Klestova Zinaida S..
• Natalia Matvienko – “Biological properties and indication of anemia virus infeketsiynoi horses” (03.00.05 – Virology) (2002) Supervisor: Doctor of Veterinary Science, Professor Anatoly Pavlovich Starcheus.
• Nikolai Sityuk – “Determination of the optimal timing of vaccination of pigs against classical swine fever, derived from immune sows” (16.00.03 – veterinary microbiology and virology) (2004). Supervisor: Candidate of Veterinary Sciences Shikov Alexander Tihonovich.
• Lyudmila Bezditko – “Gastro-intestinal infections in calves farms disadvantaged by bovine leukemia etiology, pathogenesis, therapy) (16.00.03 – Veterinary Microbiology, epizootiology, infectious diseases and immunology) (2010). Supervisor: Doctor of Veterinary Sciences, Senior Researcher Klestova Zinaida S..
• Paul Yul’evich Grubich – “Development of diagnostics and serological monitoring rhinopneumonia horses in Ukraine” (16.00.03 -veterinarna microbiology, epizootiology, infectious diseases and immunology) (2010). Supervisor: Doctor of Veterinary Sciences, Senior Researcher Vitaly A. Sinitsin.
• Vlasta Vyacheslavovna Kulikova – “Experimental proof identification of the causative viral arteritis of horses on the polymerase chain reaction” (16.00.03 – Veterinary Microbiology, epizootiology, infectious diseases and immunology) (2011). Supervisor: Doctor of Veterinary Sciences, Senior Researcher Vitaly A. Sinitsin.
• Olga Stepanovna EPL - “Antiviral action of chemical compounds on model horses herpesvirus type 1″ (16.00.03 – Veterinary Microbiology, epizootiology, infectious diseases and immunology) (2011). Supervisor: Doctor of Veterinary Sciences, Senior Researcher Klestova ZS

The most significant development, embedded in veterinary practice:

Invention Certificate, Patents

The most significant development, embedded in veterinary practice

1980

• Invention Certificate 815991 USSR MKI A 61 K 35/78 C 12 N 7/00. Adjuvant for biologics / AA Gusev, Ants VK, Onufriyeva VP, Sobko AI Lobanov SB, Shvetsov YF.; Registered. 11.21.80.

1981

• Invention Certificate 852210 USSR MKI A 01 C 3/02 Method manure storage / Sobko AI,Orobinsky II (USSR). -2877391 / 30-15; Stated 12.21.79; publ. 7.4.81, Bull. 29.
• Certificate for efficiency suggestion number 652 from 03.08.81. immunoglobulin Nonspecific blood of cattle and pigs / Sobko AI,
• Ants VK, Shinkarev F.S.; Registered. 3.8.81.

1982

• Invention Certificate 926805 USSR MKI A 61 K 31/73. Adjuvant / Ants VK, Bogomaz VI, Sobko AI, Chuyko AA Voronin EF, Ogenko VM (USSR). -3002587 / 30-15; Stated 11.10.80; reg. 7.1.82.

1983

• Invention Certificate 1027868 USSR MKI A 61 K 47/00; With 12 N 7/00 adjuvant / Ants VK, Bogomaz VI, Sobko AI, Chubko AA Voronin EF, Ogenko VM (USSR). – 3244812 / 30-15; Stated 05.02.81; reg. 3.5.83.
• Invention Certificate 1025020 USSR MKI A 61 K 39/00; With 12 N 7/00 strain Enterovirus suis -With Univ used to make virusvaktsiny against enteroviral gastroenteritis and diagnostic products for the neutralization reaction / Sobko AI, Vabishchevich F.S, Starcheus AP Tatskaya VN (USSR). – 3395276 / 30-15; Stated 10.02.82; reg. 02.22.83.
• Invention Certificate 1067820 USSR MKI C 12 N 9/00. Method of growing virus / AI Sobko, GH Subaeva (USSR). – 3315276 / 30-15; Stated 07.06.81; reg. 15.09.83.
• Invention Certificate 1086809 USSR MKI C 12 N 7/00, A 61 K 9/12 method dіagnostiki enteroviral gastroenteritis of pigs / Sobko AI, Starcheus AP Priskoka VA, Blotskaya AF, Polulyakh B. I. Sidorov IV (USSR). -3483612 / 30-15; Stated 08.17.82; reg. 12.15.83.
• Invention Certificate 1080477 USSR MKI 3 12 N 7/00. A method for preparing antigen for diagnosis of enteric coronaviruses and gastroenteritis of pigs in the reaction long complement fixation / Sobko AI, Starcheus AP Priskoka VA, Blotskaya AF (USSR). – 3455184 / 30-15; Stated 04.22.82; reg. 11.15.83

1984

• Invention Certificate 1102098 USSR MKI A 61 K 9/50. A method for producing a vaccine formulation for oral administration / AP Lazarev, AI Sobko, AV Makarenko, VG Zvozchik, VP Rizhenko, A-RA. Kucheryavenko, AA Kucheryavenko A, A. (USSR). – 3507692 / 30-15; Stated 29.10.82; reg. 3.7.84.
• Invention Certificate 1124589 USSR MKI C 12 N 7/00. A method for producing rotavirus / Sobko AI, Cupcini LG (USSR). – 3632009 / 30-15; Stated 05.18.83; reg. 15.07.84.

1985

• Invention Certificate 1156291 USSR MKI A 61 K 9/52. A method for producing a drug for oral administration / AP Lazarev, Sobko A., K. Martinek, LevashovAV Berezin, IV, Kucheryavenko AA (USSR). – 3654607 / 30-15; stated 21/10/83; reg. 01.15.85.
• Invention Certificate 1172108 USSR MKI A 61 K 9/52. A method of producing a preparation for oral / Sobko AI, Lazarev A., K. Martinek, Levashov
• AV Berezin, IV, Kucheryavenko AA, AJ Karas, Volynets AA, Makarenko AV (USSR). – 3654605 / 30-15; Stated 21.10.83; zaregistr.08.04.85.
• Invention Certificate 1182708 USSR MKI A 61 K 9/52. A method of producing a preparation for oral / Sobko AI, Lazarev A., K. Martinek, Levashov__AV Berezin, IV, Kucheryavenko AA, AA Volynets, KarasO.Ya., SpivakN.Ya. (USSR). -3654602 / 30-15; Stated 21.10.83; reg. 6.1.85.
• Invention Certificate 1223454 USSR MKI 4 A 61 K 9/52. A method of producing a preparation for Invention Certificate
• oral / AP Lazarev, K. Martinek, Sobko AI, Kucheryavenko AA (USSR). -3654606 / 30-15; Stated 21.10.83; zaregistr.08.12.85.
• Invention Certificate 1223632 USSR MKI 4 C 12 N 11/00. A process for preparing immobilized preparations / AP Lazarev, Sobko AI, AV Levashov, K. Martinek, Berezin, IV (USSR). – 3654896 / 28-14; Stated 21.10.83; reg. 12.08.85.

1987

• Certificate for rationalization proposal № 439-11 / 2113-565.
• A method of increasing the specific activity of diagnostic sera using kremnezemovym / Sobko AI, Starcheus AP Bogomaz VI, Chuyko AA, Ogenko VM, Vabishchevich FS, Volodya NA .; Registered. 01.15.87.
• Invention Certificate 1309365 USSR MKI 4 A 61 K 39/12. A method for isolating viral particles / Sobko AI, Priskoka VA (USSR). – 3779177 / 28-14; Stated 08.09.84; reg. 1.8.87.
• Invention Certificate 1349045 USSR MKI 4 A 61 K 39/225. A method for producing diagnostic antiviral immune serum / Sobko AI, Starcheus AP Chuyko AA, Likalov VK, Bogomaz VI, Country MV, Ogenko VM, Khimich GF, Vabishchevich FS (USSR). – 3834418 / 28-13; Stated 01.03.85; zaregistr.01.07.87.
• Invention Certificate 1356290 USSR MKI 4 A 61 K 39/225. A method for producing a vaccine against rotavirus gastroenteritis of pigs / Kucheryavenko AA Sobko AI, Kucheryavenko AA, AP Lazarev (USSR). – 3959833 / 30-13; Stated 10.01.85; zarigistr. 1.8.87.
• Invention Certificate 1361765 USSR MKI 4 A 61 K 39/39. Adjuvant / Sobko AI, Tatskaya VN, VG Kwacha, Resnick NA. (USSR). – 4060970 / 30-13; Stated 02.25.86; reg. 22.08.87.
• Invention Certificate 1387407 USSR MKI 4 C 12 N 7/00. The method of cultivation and enteric coronavirus / Sobko AI, Priskoka VA, Borisevich BB, manga KV (USSR). – 4143774 / 30-13; Stated 06.24.86; reg. 08.12.87.
• Invention Certificate 1361764 USSR MKI 4 A 61 K 39/225. A method for producing a vaccine against swine gastroenteritis coronavirus / Kucheryavenko AA Sobko AI, Kucheryavenko AA, AP Lazarev (USSR). – 3959236 / 30-13; Stated 10.01.85; reg. 22.08.87.

1988

• Invention Certificate 1399944 USSR MKI 4 A 61 K 39/42. A method for producing the antibody preparation – diagnosticum / Sobko AI, Starcheus AP, AA Chuyko pickup VN, Bogomaz V., Ogenko VM (USSR). -4129857 / 30-13; stated 01.07.86; reg. 2.1.88.
• Invention Certificate 1405149 USSR MKI 4 A 61 K 39/42. A method of producing an antigen and antibody diagnostics transmissible gastroenteritis of pigs / Sobko AI, Starcheus AP, colic VM, VM Molodkin, Sinitsyn VA, Vostryuhina AA, Wolf MP . (USSR). – 4150259 / 30-13; Stated 01.07.86; reg. 02.22.88. AS 1398131 USSR.
• Invention Certificate 1422427 USSR. sposobpolucheniya associated coronary vaccine, rotavirus and enterovirus gastroenteritis of pigs / Kucheryavenko AA Sobko AI, Kucheryavenko AA, AP Lazarev (USSR). – 3961858 / 30-13; Stated 10.01.85; reg. 8.5.88.
• Invention Certificate 1447326 USSR MKI 4 A 01 K 1/03. Device for feeding animals gnotobiotic / Sobko AI, Shabliy VJ, Ovchinnikov II,
• Nosov VA Pavlov, EG (USSR). – 4283958 / 30-16; Stated 06.15.87; publ. 12.30.88. Bull. 48.
• Invention Certificate 1466211 USSR MKI A 61 K 31/415 1 – (b1 – Oxy – 21 -oksageksil) – 2 – triftormetiltiometilbenzamidazol having activity against transmissible gastroenteritis virus of swine / Yavorsky AE, Bogomaz IV, Sobko AI ., Tatskaya VN, VG Kwacha, Florentiev VL.; Registered. 15.11.88.

1989

• Invention Certificate 1552421 USSR MKI 5 A 61 K 39/39, 39/125. a method of stimulating the immunological status of the animals and the immune response to antigens / Sobko AI, Tatskaya VN, VG Kwacha (USSR). – 4456171 / 30-13; stated 07.13.88; reg. 22.11.89.

1990

• Invention Certificate 1575713 USSR MKI 5 G 01 N 33/53. way to assess the immunological status of the animals / W. Kwacha, AI Sobko, EI Zlischeva, AJ Shurigin, JG Rasulov (USSR). -4453792 / 30-13; Stated 05.07.88; reg. 1.3.90.

1991

• Invention Certificate 1699049 USSR. A method for producing interferon / Spivak NY, guts Ya Sobko AI, AJ Karas, Ivanenko VK, Gapova LA, AP Lazarev, Kucheryavenko AA, Romanov NN (USSR). – 4695444; Stated 05.18.89; reg. 15.10.91.
• Invention Certificate 1697317 USSR MKI 5 A 61 K 37/66. Method Formulation
• interferon / Sobko AI, AJ Karas, Spivak N. Lazarev, AP, AA Kucheryavenko, Gylling EV, Romanov NN (USSR). – 3749272/15; stated 06.03.84; reg. 08.10.91.

1994

• Patent 2419 Ukraine, MKI 5 G 01 N 83/53. Way to assess the immune status of animals / VG Kwacha, AI Sobko, EL. Zlischeva, AJ Shurigin, JG Rasulov (Ukraine). – 4453792 / SU; Stated 28.02.94; publ. 26.12.94, Bul. 5
• Patent 2421 Ukraine, MKI A 61 K 39/39. Adjuvant / AI Sobko, V.N.Tatska,V.G.Kvachov, S.R.Reznik (Ukraine). – 4060970 / SU; Stated 02.15.94; publ. 26.12.94, Bul. 5-1.
• Patent 2417 Ukraine, MKI 5 A 61 K 39/39, and 61 K 39/125. a method of stimulating the immune status of animals and the immune response to an antigen /A.I.SOBKO, V.N.Tatska, V.G.Kvachov (Ukraine). – 4456171 / SU; Stated 02.15.94; publ. 26.12.94, Bul. 5-1.
• Patent of Ukraine in 2493, IPC A 61 K 39/00, C 12 N 7 00. Strain Enterovirus suis – un C, which is used for the manufacture of virus vaccine against enterovirus gastroenteritis of pigs and diagnostic agents for the neutralization / A.I.SOBKO, B .N.Tatska, F.S.Vabischevich, A.P.Starcheus (Ukraine). – 3395276 / SU; Stated 28.02.94; publ. 26.12.94, Bul. 5-1.

1997

• Patent 20500 Ukraine, MKI 5 A 61 K 35/00. A method of virus inactivation / Z.S.Klestova, A.I.Sobko, V.P.Rizhenko (Ukraine). – 95052507; stated 25.05.95; registered 15.07.97.
• Patent 20529 Ukraine, MKI 6 C 12 N 15/00. A method for producing rapid model for testing drugs in antimutagenic viral genome damage the cells of pigs / Z.S.Klestova, A.I.Sobko, V.P.Rizhenko (Ukraine). – 95073164 / SU; Stated 07.06.95; registered. 15.07.97.
• «Guidelines for detection and prevention of cell cultures contamination by mycoplasma and viruses” (K., 1984).
• «A method of pigs diagnosing by enterovirus gastroenteritis ” Invention Certificate №108030 9 (1983).
• «How to prepare antigen for the diagnosis of enteric coronaviruses and gastroenteritis of pigs in the reaction long n” complement fixation Invention Certificate №1080477 (1983).
• «A method for isolating viral particles” Invention Certificate №1309365 (1987).
• «A method of preparing antigens and sera for comprehensive diagnosis of viral gastroenteritis in pigs neutralization” (1987).
• «A method of producing a diagnostic antisera against transmissible gastroenteritis virus of swine” – Invention Certificate №132602 (1987).
• «Guidelines for the formulation of long-term complement fixation reaction for detection and identification of enteroviruses pigs” Moscow, 1987).
• «Recommendations for selection, delivery and storage of pathological material for the diagnosis of viral gastroenteritis of pigs” (K., 1987).
• «Guidelines to determine the antigenic relationship, differences and dominant virus in serological tests” (K., 1987).
• «Guidelines for the cultivation of transplantable cell culture lines sung-M, PSP, KHS, KTP” (K., 1987).
• «Guidelines for the diagnosis, prevention and control measures mixed (coronary, rotavirus and enterovirus) infections of pigs” (K., 1988).
• «The method of cultivation of enteric and coronaviruses”. Invention Certificate №1387407 (1988).
• Research Materials “Set of diagnostic tests for pigs transmissible gastroenteritis in the neutralization reaction” Technical Conditions 46.1526-94.
• Research Materials “Set of diagnostic tests for pigs enteroviral gastroenteritis in the neutralization reaction”. Technical Conditions 46 1525-94.
• Research Materials “Set of diagnostic tests for pigs enteroviral gastroenteritis in the reaction of prolonged complement fixation”. Technical Conditions 46.15
• Research Materials ” Dry complement for the reaction of complement fixation (RCF) “. Technical Conditions 46,15.27-94.
• Research Materials ” Hemolytic serum for reaction of complement fixation (RCF)”. Technical Conditions 46.15.24-94.
• Research Materials ” Virus vaccine against pigs transmissible gastroenteritis from lyophilized strain P 1439/81”. Technical Conditions 10.07.124-89.
• Research Materials ” Virus vaccine against pigs enteroviral gastroenteritis from strain Un of 6^th serogroup “. Technical Conditions 46.15.077-95.
• Research Materials ” Virus vaccine against pigs enteroviral gastroenteritis from strain P15 / 91 of 7th serogroup” Technical Conditions 19024865.003-93.
• Research Materials “Virus vaccine against pigs enteroviral gastroenteritis from strain B 386/79 of 8^th serogroup”. Technical Conditions 46.15.078-95.
• Research Materials “Freeze-dried virus vaccine of strain K against pigs rotavirus disease for parenteral use”.Technical Conditions 1902.48.65.004-93.
• Research Materials “Kit for the diagnostics of equine influenza in the hemagglutination inhibition (HI)”. Technical Conditions 46.15.372-99.
• Guidelines for conducting diagnostic tests for horses influenza investigation using a modified hemagglutination inhibition (1996).
• «A method for reducing infectious properties of horses herpesvirus “, Invention Certificate №54630 (2010).
• «Strain of Enterovirus suis». Invention Certificate №1025020 (1982).
• «A method of rotavirus producing”. Invention Certificate №1124589 (1983).
• «Set of diagnostics of horses rhinopneumonia for neutralization reaction”. Technical Conditions of Ukraine 24.4-055108.30-078: 2006.
• «Set of diagnostics of horses viral arteritis for neutralization reaction. Technical Conditions of Ukraine 24.4-0551083-077: 2006
• «Set of diagnostic tests for classical swine fever by ELISA method. Technical Conditions of Ukraine 46.15.045-94.
• «Set of diagnostic tests for swine erysipelas by immunoassay”. Technical Conditions 46.15.046-94.
• «Set of diagnostic tests for trichinosis by immunosorbent assay. Technical Conditions of Ukraine 46.15.235-97.
• «Set of diagnostic tests for swine transmissible gastroenteritis by immunosorbent assay. Technical Conditions 46.15.200-97.
• «Set of diagnostics of pigs enteroviral gastroenteritis and disease Teschen for enzyme immunoassay. Technical Conditions of Ukraine 46.15.044-94.
• «Set of diagnostics of viral horses arteritis for immunoassay. TU U 24.4-05510830-081: 2006.
• «Set of horses influenza diagnostics for immunoassay. Technical Conditions of Ukraine 24.4-05510830-080: 2006.
• Immunoassay test “Dia VLV-Ab». Technical Conditions of Ukraine 24.4-242651866-723-2004. (In collaboration with the Laboratory of Molecular Biology).
• Test system «EHV1 PCR tests” to identify DNA horses herpesvirus type 1 by polymerase chain reaction (PCR) Technical Conditions of Ukraine 24.2-05510830-073: 2006 (In collaboration with the Laboratory of Molecular Biology).
• Test system «EAV-3T-PCR test” to detect viral RNA of equine arteritis by reverse transcriptase polymerase chain reaction (RT-PCR) Technical Conditions of Ukraine 24.2-05510830-069: 2006. (In collaboration with the Laboratory of Molecular Biology ).
• «Guidelines on application of neutralization reaction micromethods in cell culture and diffuse precipitation reaction in agar gels for detection of antibodies to horses rhinopneumonia virus ” (2009).
• State Standard of Ukraine “Veterinary medicine. Methods of horses rhinopneumonia laboratory diagnosis” (2009).
• «Method of reaction procession to indicate antigen of Equine Infection Anemia virus” (2001).
• «Guidelines for the identification, accounting chromosome aberrations of pig cells at viral infections” (2005).
• «Guidelines for the identification and monitoring of substances antiviral properties in vitro» (2010).
• «Accelerated method for the diagnosis of CSF using rabbit antiswine immunoglobulin labeled with horseradish peroxidase” –
• Patent of Ukraine № 58868 (2003).
• «Test system based on micromethod of neutralization reaction for the diagnosis of horses rhinopneumonia ” – Patent of Ukraine №25282 (2007).
• «Test system based on immunoassay for the in vivo diagnosis of trichinosis”;
• «Express-ELISA method for the detection of specific antibodies in serum to Teschen disease virus “;
• «Mean of detecting specific antibodies to bovine leukemia by ELISA method “;
• «Test system based on micromethods of neutralization reaction for the diagnosis of equine arteritis”;
• «Method biochemical assessment of immune antibodies level in the infected animals”;
• «Biochemical assessment method of disinfectant preparation toxicity in serum and blood by AWM (Average Weight Molecules) level” and others.

Historical photo chronicle Institute

Heads of institutions in different years

Its first director was appointed corresponding member of the Academy of Agricultural Sciences and UAAN, Honored Scientist of Ukraine, Professor, Doctor of Veterinary Sciences, Anatoly Sobko, who for more than 20 years as head of the Institute and was head of the laboratory of virology (1977-1997.)

From 1997 to 2011, was director of the Corresponding Member of NAAS of Ukraine, candidate of veterinary sciences Obrazhey Anatoly.

In 2012 he was appointed director of the institute doctor of veterinary sciences, senior researcher NYCHYK SERGHIY.

Over the years, the Institute of Veterinary Medicine, scientists worked such as:

C.v.s. Atrazheva T.F.; C.v.s. Bozhko K ; C.v.s. Vabishchevich F.S.; D.v.s. Prof .; Volynets L.K.; C.v.s. Grishok LP.; C.v.s. Glavatsky VP.; C.v.s.. Zhukovsky AM.; Academician NAAS, D.v.s. Prof. Blizzard A.I.; C.v.s.. Zvozchik V.G.; C.v.s. Karas AY.; MD Kwacha V.G.; C.v.s. Kolos YA.; С.v.s. Korzunenko A.F.; Ph.D. Kucheryavenko A.A.; Ph.D. Kupchinskaya L.G.; D.v.s. Lazarev AP.; С.v.s. Kamenshik M.A. ;    C.v.s. Muraviov V.K. ; C.v.s.. Nastenko V.D.; C.v.s. Nosov V.A.; C.v.s. Naumova NA.; D.v.s.Oksamitny M.K.; Ph.D. Ostapets M.G.; C.v.s. Pavlov EG.; D.v.s., prof. Pogrebnyak L.I; C.v.s. Polulyakh V.I.; D.v.s Priskova VA.; C.v.s..Sinitsin AY.; C.v.s.. Sobko Y.A.; С.b.s. Sokirko T.A.;

D.v.s Prof . Starcheus AP.; C.v.s..Stepanok AP.; C.v.s..Subaev G.H.; C.him.s.Tairova T.M.; C.m.s.Tatska V.N.; C.v.s. Trotsenko B.L.; prof . Shabliy VY.; AT C.v.s.Shikov D.v.s., prof. Yaschenko M.F, et al.